Dwight E. Lynn
I investigate the use of cell cultures for studying diseases of insects. A cell is the smallest part of a plant or animal (including insects) that can live by itself and cell culture is the means by which cells can be removed from a living organism and still be kept alive. Once cells are in culture, they can be exposed to insect pathogens and other materials to find out how the cells respond. In this way, we can develop these agents as alternatives to chemical pesticides. Most of my current work involves studying virus diseases that can be used to kill the insects that are pests of vegetables and trees.
Recent Publications:
- Lynn, D. E. Development and characterization of insect cell lines. Cytotechnology 20: 3-11, 1996.
- Sheppard, C. A. and Lynn, D. E. Immunoreactivities for calcium signaling components and neural-like properties of a Colorado potato beetle cell line. Arch. Insect Biochem. Physiol. 33(3-4): 197-209, 1996.
- Lynn, D. E. and Shapiro, M. New cell lines from Heliothis virescens: characterization and susceptibility to baculoviruses. J. Invertebr. Pathol. 72: 276-80, 1998.
- Gundersen-Rindal, D., Lynn, D. E. and Dougherty, E. M. Transformation of lepidopteran and coleopteran insect cell lines by Glyptapanteles indiensis polydnavirus DNA. In Vitro Cell. Dev. Biol. - Animal 35(2): 111-4, 1999.
- Lynn, D. E. Comparison of cell line maintenance procedures on insect cells used for producing baculoviruses. In Vitro Cell. Dev. Biol. - Animal 35(5): 248-51, 1999.
- Lynn, D. E. Development of insect cell lines: Virus susceptibility and applicability to prawn cell culture. Meth. Cell Sci. 21: 173-81, 1999.
- Lynn, D. E. Effects of long- and short-term passage of insect cells in different culture media on baculovirus replication. J. Invertebr. Pathol. 76: 164-168, 2000
- Dougherty, E. M., McKelvey, T. A., Stoltz, D. A., Lynn, D. E. and Gundersen-Rindal, D. E. Stable insect virus-cell expression system. USA 6143565. Nov. 7, 2000. (Patent)
- Gundersen-Rindal, D., Slack, J. M. and Lynn, D. E. Transfection of Lymantria dispar insect cell lines. Meth. Cell Sci. 22(4): 253259, 2001.
- Lynn, D. E. Novel techniques to establish new insect cell lines. In Vitro Cell. Dev. Biol. - Animal 37:319-321, 2001.
- Lynn, Dwight E. 2002. Methods for Maintaining Insect Cell Cultures. 7 pp. J. Insect Sci.2.9, 2.9. Available online: http://insectscience.org/2.9
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